ZHU Yingwen^ab , HUANG Hailin^ab , LI Yanan^ab , HU Tingting^ab , HAN Biao^ab , CUO Yon^ab

( a. College of Intelligent Medicine & Biotechnology, b. Key Laboratory of Biochemistry & Molecular Biology ofGuangxi Institutions of Higher Learing, Guilin Medical University, Guilin 541199, China)

AbstractObjective To investigate the effect of epigallocatechin gallate( EGCG)coupled with mechanicalstimulation ( MS) on the proliferation and differentiation in osteoblasts. Methods 'The osteoblasts were divided intocontrol group, EGCG group, MS group, and EGCG+MS group. The proliferation of osteoblasts was detected by M'Tlassay. 'The mineralisation of osteoblasts were detected by alizarin red staining method. T'he mechanical load wasapplied to the cells by a four-point bending mechanical loading device, and the Alkaline Phosphatase ( ALP ) TestKit was used to deteet the AlP activity. The protein expressions of runt-related transcription factor-2 ( Runx-2) andcollagen type l ( Col-l) in osteoblasts were deteeted by western blot. Results EGCG at a coneentration of 30 umol/L had the most pronounced proliferative elfect on osteoblasts, with a statistically significant differenee( P<0.05 ). Thenumber of mineralised nodules in the EGCG group was greater than that in the control group. Compared with thesingle EGCG or MS groups, the AlP activity, in the EGCG+MS group was significantly inereased and the proteinexpressions of Runx-2 and Col-l in the EGCG+MS group were significantly inereased( P<0.05). Conclusion EGCG.coupled mechanical stimulation enhances the ALP activity , up-regulates the expressions of Runx-2 and Col-l proteinsin osteoblasts , and promotes osteoblasts'proliferation and differentiation.Keywords: epigallocatechin gallate ; osteoblast; mechanical stimulation; osteogenie differentiation

DOI:10.19296/j.cnki.1008-2409.2024-02-012

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